MICROSCOPY WHITE PAPERS AND CASE STUDIES

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Is A DIY Microscope For You?
Whether modifying existing instrumentation or building a new tool from the ground up, a meticulous process is as critical as the functional result.  Continue Reading..
Optical Components for SWIR Imaging
Optical Components For SWIR Imaging
This application note provides information on the benefits of SWIR imaging in harsh or low-light level conditions, and PG&O's capabilities in manufacturing optical components for SWIR imaging.  Continue Reading..
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Improving Microscopy And Biological Imaging With Backside Illuminated (BI) sCMOS Sensors
Biological labs can get better and more quantifiable image data from microscopes with sCMOS sensor-based devices.  Continue Reading..
DNA-PAINT: A Super-Resolution Microscopy Technique
DNA-PAINT: A Super-Resolution Microscopy Technique
The recently introduced super-resolution method DNA-PAINT is based on transient DNA-DNA interactions. This white paper discusses the DNA-PAINT microscopy technique and how sCMOS cameras are well suited for these kinds of measurements.  Continue Reading..
Xeva-1.7-320 Brochure: SWIR Imaging Camera for Advanced Research
Xenics’ Xeva-1.7-320 digital camera combines a thermo-electrically cooled InGaAs detector head and the control and communication electronics in a compact housing. Single or three-stage Peltier cooling reduces the noise and dark current for enhanced low light-level imaging. The Xeva-1.7-320 combines standard (up to 1.7 µm) InGaAs detector arrays with various speed versions: 60 Hz, 100 Hz and 350 Hz. This allows you to choose the most...  Continue Reading..
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Micromirror TIRF Microscopy: Technique And Applications
Total internal reflection fluorescence (TIRF) microscopy is used to watch biological processes unfold in real time. Taking advantage of the ability to label individual molecules with different colors of fluorescent tags, TIRF microscopy lets scientists view the complex molecular assemblies that govern cellular processes.  Continue Reading..
Understanding Interference Tilted Excitation (LITE) Microscopy
Understanding Interference Tilted Excitation (LITE) Microscopy
When the detection of a cellular or subcellular structure requires a high NA detection objective, conventional fluorescence microscopy and light sheet fluorescence microscopy (LSFM) encounter challenges. Lateral interference tilted excitation (LITE) microscopy overcomes this problem.  Continue Reading..

MICROSCOPY PRODUCTS

The pco.edge 5.5 sCMOS camera operates with streaming via a 10G FOL (Camera Link HS). It allows for direct processing of large data transfers on a computer. This high-performance data interface makes it easy to securely bridge distances of more than 10 km.

The pco.edge 4.2 sCMOS camera operates with streaming via a 10G FOL (Camera Link HS). It allows for direct processing of large data transfers on a computer. This high-performance data interface makes it easy to securely bridge distances of more than 10 km.

The pco.edge 4.2 bi UV is a compact, cooled, back illuminated sCMOS camera designed to work inside the ultraviolet (UV) wavelengths. A specialized input window inside the camera achieves high QE within the UV range. Based on the compact pco.edge series with back illuminated sensor technology, this camera is perfect for demanding lighting conditions, even without active cooling.

The pco.panda 4.2 bi UV is a compact, back illuminated sCMOS camera designed to work inside the ultraviolet (UV) wavelengths. A specialized input window inside the camera achieves high QE within the UV range. Based on the compact pco.panda series with back illuminated sensor technology, this camera is perfect for demanding lighting conditions, even without active cooling.

PCO offers the pco.edge 26 sCMOS camera with 5120 x 5120 pixel resolution and a 2.5 x 2.5 µm² pixel size for achieving the maximum level of spatial resolution for many applications, including microscopy imaging with low magnification.

The pco.edge 4.2 bi is a back illuminated camera sensor with high resolution and a 6.5 x 6.5 μm² pixel size for high-quality images with quantum efficiency up to 95%. The pco.edge 4.2 bi incorporates a powerful USB 3.1 interface, and a flexible cooling system allowing the use of air or water to cool the sensor down to -25 °C. At this temperature, the dark current is reduced to 0.2 e-/pixel/s. 

PCO introduces its latest camera innovation: the pco.panda 4.2bi, a state-of-the-art, back illuminated sCMOS camera providing outstanding quantum efficiency, up to 95%. It is ideal for demanding lighting conditions, even without active cooling, in countless applications in microscopy and life science.

The new Ophir® PD300-MS microscope slide power meter designed to make accurate measurements of emitted light from fluorescent microscopes. Measuring power levels of high numerical aperture (NA) objectives, from 5 µW to 1 W, this power meter can be used with a wide range of light sources in such applications as photo-activation or photo-bleaching.

MICROSCOPY SUPPLIERS

BaySpec, Inc., founded in 1999 with 100% manufacturing in the USA (San Jose, California), is a vertically integrated spectral sensing company. The company designs, manufactures and markets advanced spectral instruments, including UV-VIS-NIR-SWIR spectrometers, benchtop and portable NIR/SWIR and Raman analyzers, confocal Raman microscopes, hyperspectral imagers, mass spectrometers, and OEM spectral engines and components, for the R&D, biomedical, pharmaceuticals, chemical, food, semiconductor, health monitoring, human & animal medical devices, and the optical telecommunications industries.

Canon U.S.A., Inc., is a leading provider of business-to-business, industrial and consumer digital and optical imaging solutions to the United States, Latin America and the Caribbean (excluding Mexico) markets with an increasing intent for more customized, localized products.

Infrared Cameras|Infrared Imaging Subsystems|Thermal Cameras|Infrared Camera Subsystems|Ruggedized Infrared Cameras|Visual Inspection, Thermal|Infrared Surveillance|Video Inspection Systems, Thermal|Infrared Detectors|Thermal Sensors|Night Vision Cameras|Night Vision Systems|Detectors, Infrared|Detectors, Heat|Infrared Imagers|Infrared Equipment|Infrared Sensors|Security Systems, Infrared|Thermal Imaging Systems|Military Infrared Sensors|Integrated Detective Assemblies, Infrared|Optical Inspection Systems,Thermal|Thermal Camera Systems|Night Vision Components|Infrared Video Cameras|Data Acquisition Software,Infrared|Focal Plane Arrays|Machine Vision Systems, Infrared|Infrared Industrial Detectors|Infrared Inspection Systems|Automated Inspection, Infrared

Gooch & Housego provides complete optical system design, engineering and manufacturing services to the aerospace & defense, industrial, life sciences and scientific research sectors, based upon the following key enabling photonic technologies: Acousto-Optics, Crystal Optics Electro-Optics, Fiber Optics and photonic packaging, Non-Linear Optics, Precision Optics including optical polishing and coatings, as well as light measurement instrumentation and services, spectral Imaging & synthesis.

Photomultiplier Tubes|Arc Lamp|Photo Multiplier|Spectroscopy Systems

Iridian Spectral Technologies is a diversified optical filter manufacturer that is an international leader in filter design and manufacture especially for application in the fields of fiber optic communications, optical spectroscopy, 3D entertainment, and in aerospace. Iridian Spectral Technologies was ranked as one of the top 150 high technology companies in Canada in the 2011 and 2012 Branham300 rankings and won the 2010 Canada Export Achievement Award for the province of Ontario sponsored by Profit magazine together with Export Development Corporation (EDC). Iridian Spectral Technologies is a global supplier with distributors in many countries particularly in Asia.

Mad City Labs, Inc. is the leading US manufacturer of precision piezo based nanopositioning stages and nano-instrumenation.  We manufacture high performance motion control instrumentation including our innovative closed loop nanopositioning systems, high stability microscopy platforms, low cost probe microscopy and metrology solutions.  Our instruments are suitable for super resolution microscopy, metrology, imaging, photonics, interferometry and astronomy.

PCO-TECH Inc., founded in 1986, is a manufacturing, sales and marketing organization offering photonic instrumentation and complete systems throughout the world

Photron was founded in 1974 to provide manufacturing, sales and service of professional film and video equipment and photo-instrumentation

PI (Physik Instrumente) provides the world’s broadest range of precision motion technologies for both micro- and nano-precision applications. PI is the world’s first and largest manufacturer of piezoelectric nanopositioning technology.  Started 4 decades ago as a supplier to research labs and universities, the company has grown into a business of 700+ employees and more than $100 million in global sales.

For over 30 years, Precision Optics Corporation has provided state-of-the-art optical designs, components and complete optical systems for medical, biomedical, military (ITAR complainant) and industrial applications. As next generation techniques in these areas have advanced to use smaller and less invasive tools and equipment, Precision Optics responded with microprecision™ optical technology to provide components, assemblies, and products with sizes on the order of one millimeter and smaller.

Raptor Photonics Limited, founded in 2006, is a global leader and manufacturer of high performance, industrial-grade and rugged low light digital & analogue cameras. Raptor specialises in complete cameras and core engine solutions using EMCCD, Scientific CMOS, SWIR and Vis-SWIR sensor technology.

UTC Aerospace Systems (Sensors Unlimited Products), is the world’s leading manufacturer of indium gallium arsenide (InGaAs)-based imaging products. As renowned experts in shortwave infrared (SWIR) imaging, Sensors Unlimited, Inc. introduced the world to the concept of imaging in the shortwave IR spectrum with simple, compact, and uncooled cameras.

Xenics is a well established and rapidly growing high-tech company about to enter exciting new fields of IR products and applications, serving the markets with excellent products and a strong technology background.

Supplier of precision optics including extreme precision aspheres and freeform, meter-class flats and windows in common and exotic materials. Full electro-mechanical-optical design/prototype and manufacturing capabilities for medical, defense, military, ISR, semiconductor and industrial technology research.

MICROSCOPY NEWS

  • Excelitas To Highlight Fluorescence Illumination Solutions For Microscopy At Biophysical Society 2019

    Excelitas Technologies Corp., a global technology leader delivering innovative, customized photonic solutions, will showcase its broad offering of X-Cite fluorescence illumination products for microscopy and debut its new white light LED light source at the 63rd Annual Meeting of the Biophysical Society.

  • New Ophir® PD300-MS Power Meter For Fluorescence Microscopy Measures High NA Microscope Objectives

    MKS Instruments, Inc., a global provider of technologies that enable advanced processes and improve productivity, has announced the Ophir® PD300-MS microscope slide power meter for accurate measurements of light emitted from fluorescence microscopes. The PD300-MS measures power levels of high numerical aperture (NA) objectives, from 5µW to 1W. A special filter provides low angular dependence, enabling higher accuracy measurements. NIST-traceable calibration allows for measurement of wavelengths from 350nm to 1100nm. The power meter is designed for use with a wide range of light sources in such applications as photoactivation or photobleaching. To accommodate limited space, it has the same footprint as a standard microscope slide. The PD300-MS can be used at the sample plane in air or with water/oil immersion objectives without damage.

  • Powerful New Imaging Method Reveals In Detail How Particles Move In Solution

    New research published in Nature Methods will dramatically improve how scientists “see inside” molecular structures in solution, allowing for much more precise ways to image data in various fields, from astronomy to drug discovery.

  • Rice Team Designs Lens-Free Fluorescent Microscope

    Lenses are no longer necessary for some microscopes, according to Rice University engineers developing FlatScope, a thin fluorescent microscope whose abilities promise to surpass those of old-school devices.

  • Super-Resolution Microscopy In Both Space And Time

    In a breakthrough for biological imaging, EPFL scientists have developed the first microscope platform that can perform super-resolution spatial and temporal imaging, capturing unprecedented views inside living cells. The landmark paper is published in Nature Photonics.

  • Leti Develops Lens-Free, Point-Of-Care System For Diagnosing Spinal Meningitis

    Leti, a research institute at CEA Tech, has invented a lens-free microscope technology that provides point-of-care diagnosis for spinal meningitis. Outlined in a paper presented at Photonics West, the new technology provides immediate results and eliminates errors in counting white blood cells (leukocytes) in cerebrospinal fluid, which is required to diagnose the infection.

  • Researchers Achieve Resolutions In The 30 Nanometre Range

    Objects smaller than half the wavelength of the utilised light cannot be viewed with the aid of traditional light microscopes. A research team from Ruhr-Universität Bochum (RUB) and the University of Basel has succeeded in enhancing the resolution for microscopic analysis of solid state systems to 1/31 of the wavelength of the utilised light.

  • Magnetic Fields Of Bacterial Cells, Nano-Objects Mapped Using Electron Microscopy

    A research team led by a scientist from the U.S. Department of Energy’s Ames Laboratory has demonstrated for the first time that the magnetic fields of bacterial cells and magnetic nano-objects in liquid can be studied at high resolution using electron microscopy.

  • Hollow Optical Antennas For Superior Photon Creation And Control

    Scientists at Julius-Maximilians-Universität (JMU) Würzburg in Bavaria, Germany, have introduced a novel plasmonic cavity antenna design that enhances the creation and control of photons emitted by atoms and molecules.

  • Novel Lenses Enable X-Ray Microscopy With Record Resolution

    Scientists have developed novel lenses that enable X-ray microscopy with record resolution in the nanometre regime. Using new materials, the research team led by DESY scientist Saša Bajt from the Center for Free-Electron Laser Science (CFEL) has perfected the design of specialised X-ray optics and achieved a focus spot size with a diameter of less than ten nanometres.

  • Ultraviolet Microscope To Dramatically Speed Up Lab Tests

    A microscope using ultraviolet (UV) light to provide high-resolution histological images promises to significantly shorten the amount of time diagnostic tests are processed.

  • Thorlabs Releases New sCMOS Cameras

    Thorlabs has announced the release of its new 2.1 Megapixel Quantalux sCMOS Camera based on a high-performance 1e- read-noise imager. Ideal for demanding imaging applications, the monochrome sensor can image the full frame [1920 (H) x 1080 (V)] at 50 fps with 16 Bit resolution and offers a peak quantum efficiency of 61% at 600 nm.

  • Water-Repellent Nanosheet Wrapper Enhances Biological Microscopy

    Japanese scientists have developed a unique water-repellent nanomaterial that can be wrapped around biological tissue to visualize high-quality images for a longer period of time.

  • New High Power, Low Noise, White Light Source For Microscopy

    The new Mighty Light from Spectrolight is a turnkey, compact source of broadband light based on a high power tungsten-halogen lamp which is ideal for microscopy, machine vision, photovoltaic research and testing, and other diverse illumination applications.  With a nominal color temperature of 2900K, the Mighty Light provides over 2 watts of low-noise, white light output spanning the visible through near‑infrared spectral regions (400 nm ~ 2.5 µm). 

MICROSCOPY VIDEOS